DNA sequence analysis of crown gall tumor T-DNA encoding the 0.7kb transcript

نویسندگان

چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

DNA sequence analysis of crown gall tumor T-DNA encoding the 0.7 kb transcript.

Crown gall tumor formation involves integration into the plant genome of DNA sequences (the T-region) of tumor-inducing (Ti) plasmids present in Agrobacterium tumefaciens. The T-DNA of the tumor expresses several gene products. Little is known about the function or regulation of expression of the 0.7kb transcript, which represents a relatively abundant T-DNA transcript in octopine-type tumors. ...

متن کامل

Unusual plasmid DNA organization in an octopine crown gall tumor.

A cloned tobacco crown gall tumor, 1595501, incited by A. tumefaciens strain 15955 was studied. Molecular analysis of the organization of the T-DNA by means of Southern transfer and hybridization techniques indicated that the 1595501 tumor has about 10 copies of TL DNA, five of which are complete TL DNA, whereas most octopine tumors have only one to two copies of complete TL DNA. Hybridization ...

متن کامل

Recovery of crown-gall tumor cells.

nant animal cells are distinguished from healthy or inflaznmstory cells. In emphasizing the perma nency of the cellular alteration, Kidd (6) states that “Cancercells remain such until they die . . they never turn back en masse towards the benign or normal state. . . .“The inability to accomplish a reversal of malignant to normal cells under con trolled conditions has, however, served as a s...

متن کامل

Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors.

Renaturation kinetics of labeled Agrobacterium tumefaciens DNA are not influenced by addition of 10(4)-fold excess of crown gall tumor DNA. Reconstruction experiments demonstrated that 0.01% added bacterial DNA produces a detectable increase in rate of renaturation of labeled DNA. Crown gall tumor DNA therefore cannot contain as much as 0.01% A. tumefaciens DNA (one entire bacterial genome per ...

متن کامل

DNA Encoding for Splice Site Prediction in Large DNA Sequence

Splice site prediction in the pre-mRNA is a very important task for understanding gene structure and its function. To predict splice sites, SVM (support vector machine) based classification technique is frequently used because of its classification accuracy. High classification accuracy of SVM largely depends on DNA encoding method for feature extraction of DNA sequences. However, existing enco...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Nucleic Acids Research

سال: 1984

ISSN: 0305-1048,1362-4962

DOI: 10.1093/nar/12.5.2317